Biochemistry (M.Sc.)

These pages are not displaying properly because the Compatibility View in your Internet Explorer is enabled. We suggest that you remove 'fu-berlin.de' from your list of sites that have Compatibility View enabled.

  1. In Internet Explorer, press the 'Alt' key to display the Menu bar, or press and hold the address bar and select 'Menu bar'.
  2. Click 'Tools' and select 'Compatibility View settings'.
  3. Select 'fu-berlin.de' under 'Websites you've added to Compatibility View'.
  4. Click 'Remove'.

Functional Coupling in Gene Expression and Gene Regulation

There is extensive functional crosstalk among the molecular machinery responsible for genome replication/maintenance, gene expression and gene regulation. An active area of molecular biological research is concerned with the question of how genome organization, transcription, pre-mRNA processing as well as nuclear export, cytoplasmic localization, translation and degradation of mRNAs are functionally coupled via non-coding RNAs, RNA-binding proteins and RNA-modifying enzymes, and how such coupling is influenced by environmental cues.

  • Explain with which methods you can selectively control histone modifications at a particular locus.
  • Using electrophoretic mobility shift assays, you observed that protein X binds RNA Y in vitro. How could you test whether this binding event also occurs in vivo? How can you test which other RNAs protein X associates with transcriptome-wide?
  • Explain the role of the C-terminal domain (CTD) of RNA polymerase II for the transcription process. How can you experimentally test presumed functions of the CTD?
  • Design an experiment to analyze which macromolecules are involved in the process of pre-mRNA 5’-capping. What is the function of the 5’-cap and why do bacteria not process their mRNAs? To which other gene expression/regulation processes is 5'-capping coupled and how?
  • How can you use the CRISPR/Cas9 technology in a transcriptome-wide manner to identify RNA-binding proteins that control a particular transcription event? How can you apply CRISPR/Cas9 technology to investigate further processing of individual transcripts in vivo?